Posted in | Physical Properties Testing | Particle Size Analyzers

Particle Sizer and Zeta Potential Analyzer - NanoBrook Omni

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The sizing features of NanoBrook Omni are listed below:

Fast and accurate nanoparticle size distributions
Compact bench top unit, USB connection
Multimodal and unimodal size distribution software
Range: > 0.3 nm to 10 μm
Dynamic light scattering at 173° and 90°
Temperature control, -5 °C to 110 °C
High power 35 mW diode laser
Three measurement angles: 15°, 90°, and 173°
Molecular weight determination (relative and absolute via Debye plot)
ISO 13321 and ISO 22412 compliant results

Zeta Potential

Zeta potential for the tough cases
- For zeta potential in organic solvents
- For RNA, peptides, proteins, mAb, and other biological samples
- For high-salt suspensions
- For samples near the I.E.P
- For viscous or oily media
1,000 times more sensitive than other methods
Disposable cuvettes, no alignment or contamination
Integrated automatic procedures and parameters (SOP)

Rapid, Reliable and Accurate Analysis

The latest NanoBrook Omni particle size and zeta potential analyzer incorporates everything the user may need for rapid, standard, sub-micron measurements of size and zeta potential. Grounded on the principles of Dynamic Light Scattering (DLS) for particle sizing and distribution, and on Doppler velocimetry (electrophoretic light scattering, ELS) for zeta potential, a majority of measurements take just one or two minutes. The instrument also comprises of Phase Analysis Light Scattering (PALS) measurements for samples with low mobilities (saline, PBS).

Three Scattering Angles

Measurements of traditional colloids are typically made at 90° scattering angle due to the unbiased results measured. For proteins and nanoparticles, IgG and peptides, these < 50 nm samples can be computed using the backscattering angle (173°) for ideal S/N and reproducibility of measurements. Lastly, the 15° detection angle can be chosen for increased sensitivity with aggregation measurements. Zeta potential measurements are always performed using the 15° detection angle to reduce diffusion broadening.

Principles of Operation – Sizing

Dilute suspensions, on the order of 0.0001 to 1.0% v/v are prepared, using ideal wetting and/or dispersing agents, if needed. A small ultrasonicator is occasionally beneficial in breaking up loosely-held agglomerates. At 173 sample volume may be decreased to 50 μL with a polystyrene, U-shaped, disposable cuvette and the sample is recoverable. A 90° square polystyrene or glass cells (two or three mL) are utilized, one as small as 10 μL (non-disposable). Furthermore, disposable, glass round cells with reusable Teflon stoppers are employed for aggressive solvent suspensions. In every case, only a few minutes are necessary for the sample and cell to equilibrate with the actively regulated temperature setting inside the NanoBrook Omni.

Principles of Operation – Zeta Potential

The NanoBrook Omni utilizes phase analysis light scattering to establish the electrophoretic mobility of charged, colloidal suspensions. In contrast to its cousin, Laser Doppler Velocimetry (LDV, [occasionally referred to as Laser Doppler Electrophoresis (LDE)), the PALS method does not need the application of large fields which may cause thermal issues or denaturation, because in the measurement of phase shift, the particles have to only move a fraction of their own diameter to produce favorable results. In salt concentrations up to 2 molar and with electric fields as small as 1 or 2 V/cm sufficient movement is triggered to obtain superb results. Furthermore, the Autotracking feature makes up for thermal drift.

Specifications

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Sample Type	Sizing: nano particle and colloidal-sized materials, in any non-absorbing liquid. Zeta potential: proteins, nano particle, polymer and colloidal-sized materials, suspended in any non-absorb¬ing liquid, with relative permittivity (dielectric constant) > 1.5 and viscosity < 30cP.
Size Range	Sizing: > 0.3 nm to 10 μm diameter, depending on refractive index and concentration Zeta potential: 1 nm to 100 μm, sample dependent
Mobility Range	10^-11 to 10^-7 m² /V*s
Zeta potential range	-500 mV to 500 mV, sample dependent
Maximum sample conductivity	Sizing: unlimited Zeta potential: 30 mS/cm
Sample Cells	Sizing: 1 to 3 mL disposable plastic, 50 μL disposable, 40 μL quartz flow cell, 10 μL quartz minimum Zeta potential: 180 μL, 600 μL, 1250 μL
Concentration Range	Sizing: 0.1 ppm to 50 mg/mL, depending on refractive index and concentration Zeta potential: 40% v/v, sample dependent
Signal Processing	Sizing: Dynamic Light Scattering, DLS Zeta potential: Electrophoretic & true Phase Analysis Light Scattering, ELS & PALS
Correlator	Brookhaven’s TurboCorr, multitau, research grade with 510 channels, covering the equivalent of 1010 linearly-spaced channels, 100% efficiency, real-time operation over the entire delay-time range.
Precision	Sizing: ± 1% typical Zeta potential: plus minus 3% typical
Temperature Control	-5 ° C to 110 ° C, ± 0.1 ° C, active control. No external circulator required.
Condensation Control	Purge facility using dry air, nitrogen preferred
Standard Laser	35 mW red diode laser, nominal 640 nm wavelength
Scattering Angle	173°, 90° & 15°
Data Presentation	Average & width, lognormal fit, and multimodal size distribution for sizing Doppler Frequency Shift, electro¬phoretic mobility, zeta potential using Smoluchowski, Hückel, or Henry
Compliance	ISO13321 and ISO22412 compliant results for sizing
Power Requirements	100/115/220/240 VAC, 50/60 Hz, 150 Watts
Dimensions	23.3 x 42.7 x 48.1 cm (HWD)
Weight	15 kg
Environmental Characteristics	Temperature 10 ° C to 75 ° C Humidity 0% to 95%, non-condensing
CE Certificate	Class I laser product, EN 60825-1:2001, CDRH